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Accents dessus, dessous … du flux, du flou … des sons/Farandoles de bribes, en ribambelles/Trés pétris, pête-mêle …/Appendices/Fragments distincts, fouillis d’instants/Élans, saccades, et biais du flux/Rouages d’oeillades, voire …

Fanfare: The Magazine for Serious Record Collectors

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Fanfare: The Magazine for Serious Record Collectors 2019年第4期42卷 327-328页

作者： Clarke, Colin

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Interview with Conductor Bo Holten

Fanfare: The Magazine for Serious Record Collectors

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Fanfare: The Magazine for Serious Record Collectors 2019年第3期42卷 106-110页

作者： MELTZER, KEN Fogel, Henry Clarke, Colin

The article presents an interview with conductor Bo Holten, who tells about his career development, the difference between the natural folksongs of Norway and Sweden and the national songs of Denmark and qualities of songs that appeal to those not familiar with Denmark's history and culture. He discusses the impact of the change of seasons in Denmark on the significance and emotional resonance for the Danish people.

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Shenandoah/Long Time Ago/She’s Like the Swallow/Great God Almighty/I Hunger and Thirst/Bright Morning Stars/Walden Pond/Water Night/Dirait-on/Beautiful River/Why the Caged Bird Sings/Unclouded Day

Fanfare: The Magazine for Serious Record Collectors

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Fanfare: The Magazine for Serious Record Collectors 2019年第6期42卷 50-50页

作者： Clarke, Colin

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Transcriptomic analysis of IgG4 Fc-fusion protein degradation in a panel of clonally-derived CHO cell lines using RNASeq

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Biotechnology and bioengineering 2019年第6期116卷 1556-1562页

作者： Clarke, Colin Gallagher, Clair Kelly, Ronan M. Henry, Michael Meleady, Paula Frye, Christopher C. Osborne, Matthew D. Brady, Ciaran P. Barron, Niall Clynes, Martin Natl Inst Bioproc Res & Training Fosters Ave Blackrock Dublin Ireland Dublin City Univ Natl Inst Cellular Biotechnol Dublin Ireland Eli Lilly & Co LTC North Bioproc Res & Dev Bioproc Res & Dev Indianapolis IN 46285 USA Eli Lilly SA Biotech Tech Serv Mfg Sci Irish Branch Cork Ireland Univ Coll Dublin UCD Engn & Mat Sci Ctr UCD Sch Chem & Bioproc Engn Dublin Ireland

In this study, we report an investigation of a panel of clonally-derived Chinese hamster ovary (CHO) cell lines exhibiting variability in the proportion of full-length IgG4 Fc-fusion protein produced. The recombinant protein was found to be degraded during cell culture into four shorter clipped species (three of the four cleavage sites occurred at arginine residues) and preliminary analyses suggested that a host cell enzyme was responsible for proteolysis. To identify the specific enzyme responsible, RNA sequencing was used to identify gene expression differences between the cell lines with a high and low clipping phenotype. From this analysis, six protease-encoding genes were found to be significantly upregulated in those cell lines yielding the lowest proportion of full-length IgG4 Fc-fusion protein. Four of these protease candidates were deprioritized after examination of their cleavage site specificity. The remaining enzymes, Adam19 and Furin, were found to be capable of cleavage at arginine residues, and inhibitors for both proteases were added to cell-free media to determine if the product degradation could be reduced. While the Adam19 inhibitor had no impact, Furin inhibitor I (specific for the proprotein convertase family of enzymes) was found to result in a 33-39% increase in complete IgG4 Fc-fusion protein when compared with untreated samples.